FastQCFastQC Report
Thu 2 Jun 2016
DRR015342_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015342_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11497318
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374820.3260064651599617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300020.26094781409020784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171170.14887819924611984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181050.055.9925541
GTATCAA349350.041.4952771
ATCAACG414000.034.4803163
TCAACGC415400.034.407784
CAACGCA433750.032.962635
TATCAAC441400.032.5361022
AACGCAG448100.031.97776
ACGCAGA505150.028.0599697
CGCAGAG512400.027.6273048
GTACATG333450.026.1716751
GCAGAGT553350.024.905549
TACATGG331500.024.8322372
ACATGGG335350.023.4071123
GTGGTAT76750.022.7056541
CAGAGTA538550.021.28977810-11
TATAACG8550.021.2889752
GAGTACT385700.020.88540512-13
TGGTATC80000.020.8755022
AGAGTAC511350.020.3045810-11
GAGTACA256200.019.3225021
Produced by FastQC (version 0.11.5)