FastQCFastQC Report
Thu 2 Jun 2016
DRR015342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11497318
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370510.3222577648108889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291180.25325906441832785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193830.16858714354078055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169250.056.824951
GTATCAA333050.043.0491181
ATCAACG415450.034.012273
TCAACGC417350.033.9010354
CAACGCA430350.032.908675
TATCAAC440450.032.30582
AACGCAG445450.031.9242256
GTACATG316350.027.4755381
ACGCAGA515700.027.2666767
CGCAGAG519450.026.9034398
TACATGG314500.026.1181852
ACATGGG323250.024.3478473
GCAGAGT566000.024.2617389
GTGGTAT73000.023.6759571
AGAGTAC523150.020.6089210-11
CAGAGTA546850.020.43964210-11
TGGTATC77750.020.2629572
GAGTACT403500.019.99945312-13
CATGGGG206900.019.2178124
AGTACTT417000.019.10647212-13
Produced by FastQC (version 0.11.5)