FastQCFastQC Report
Thu 2 Jun 2016
DRR015341_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015341_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15303675
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382540.24996610291318916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305460.1995991158986322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171260.1119077607176054No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222100.052.668841
GTATCAA416550.040.654581
ATCAACG494500.033.678773
TCAACGC496200.033.5448234
CAACGCA524250.031.7239675
TATCAAC531250.031.5289632
AACGCAG538800.030.9263956
ACGCAGA600000.027.4230467
GTACATG419750.027.283041
CGCAGAG604250.027.1018858
TACATGG413700.026.1668682
ACATGGG421450.024.8974363
GCAGAGT661000.024.1256089
CAGAGTA641500.020.42619910-11
AGAGTAC603100.020.14628610-11
CATGGGG267000.019.8883234
GAGTACT463900.019.41906412-13
GTGGTAT101300.018.5947861
GAGTACA312650.018.1908021
GTACTTT488300.018.178514-15
Produced by FastQC (version 0.11.5)