FastQCFastQC Report
Thu 2 Jun 2016
DRR015341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15303675
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372770.24358201543093408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288470.18849720737012515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184250.1203959179739507No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218000.049.5132261
GTATCAA404700.041.207021
TCAACGC492600.033.257284
ATCAACG493400.033.2217943
CAACGCA510800.032.0812155
AACGCAG526550.031.1628766
TATCAAC531400.031.0801352
ACGCAGA601200.027.0134627
GTACATG401050.026.9595411
CGCAGAG605450.026.6435138
TACATGG396150.025.9320182
ACATGGG408850.024.2243653
GCAGAGT668950.023.7186539
AGAGTAC605950.020.52277210-11
CAGAGTA651450.019.55386410-11
GAGTACT470250.019.21697612-13
CATGGGG262350.018.9364934
AGTACTT479600.018.61463212-13
GTACTTT492850.018.01392414-15
GTGGTAT107450.017.9520241
Produced by FastQC (version 0.11.5)