FastQCFastQC Report
Thu 2 Jun 2016
DRR015340_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015340_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276298
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358810.29227866576715555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281750.22950729935034161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155920.12700897290046234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204050.054.3626141
GTATCAA382450.041.1627771
ATCAACG453300.034.251923
TCAACGC455300.034.1514324
CAACGCA476400.032.600645
TATCAAC488700.031.8546122
AACGCAG495000.031.4214846
GTACATG425050.027.6602251
ACGCAGA555900.027.5207887
CGCAGAG560950.027.0695678
TACATGG414350.026.8188362
GTGGTAT74050.025.9292891
ACATGGG424700.025.39383
GCAGAGT605700.024.5404459
CAGAGTA584250.021.13888210-11
TGGTATC89350.020.320762
CATGGGG260000.020.1798344
GAGTACT403600.020.02085712-13
GTACTTT403000.019.66100714-15
AGAGTAC565850.019.55076610-11
Produced by FastQC (version 0.11.5)