FastQCFastQC Report
Thu 2 Jun 2016
DRR015340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276298
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347260.28287029200496766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270180.22008263403185555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168850.13754146404722337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185150.054.3438871
GTATCAA359150.042.2707941
ATCAACG442000.033.893563
TCAACGC446800.033.519264
CAACGCA459900.032.554595
TATCAAC474050.031.7813592
AACGCAG478250.031.484576
ACGCAGA551200.027.0783217
GTACATG403950.027.0641021
CGCAGAG555550.026.7354978
TACATGG392550.026.4447672
ACATGGG411150.024.243193
GCAGAGT608100.023.9222499
GTGGTAT73600.022.3615261
CAGAGTA584850.020.2676310-11
AGAGTAC569000.020.02050610-11
CATGGGG254700.019.5762424
GAGTACT408250.019.25470512-13
GTACTTT405650.019.02608714-15
AGTACTT401600.019.00134712-13
Produced by FastQC (version 0.11.5)