FastQCFastQC Report
Thu 2 Jun 2016
DRR015339_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015339_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13330865
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406530.3049539546008455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337160.2529168212265296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188780.14161121577632058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209650.055.1902241
GTATCAA407100.042.482241
ATCAACG485950.035.0495833
TCAACGC488000.034.9485784
TATCAAC512000.033.4264032
CAACGCA515850.033.1058585
AACGCAG535150.031.9626876
ACGCAGA595650.028.4106757
CGCAGAG599950.028.0171158
GTACATG406250.026.6895541
TACATGG405850.025.530172
GCAGAGT657950.024.9498549
ACATGGG414100.024.2302553
GAGTACT438350.021.40229612-13
CAGAGTA635150.021.0387710-11
AGAGTAC597900.020.54603210-11
GTGGTAT90050.020.4130691
GTACTTT478450.019.38482914-15
CATGGGG251300.019.3568614
CATGGGT110750.019.3520474
Produced by FastQC (version 0.11.5)