FastQCFastQC Report
Thu 2 Jun 2016
DRR015339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13330865
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407330.305554065696412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326140.2446502908851001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206610.15498619181876044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222250.052.0848851
GTATCAA415000.041.1648331
ATCAACG511250.032.9518663
TCAACGC513750.032.800374
CAACGCA532150.031.666245
TATCAAC539400.031.4169462
AACGCAG550050.030.6749116
ACGCAGA630500.026.6454377
CGCAGAG632050.026.3929628
GTACATG390200.025.7055071
TACATGG396750.024.190912
GCAGAGT683300.023.9658899
ACATGGG401450.022.8917773
GTGGTAT104000.021.480951
AGAGTAC626800.020.77265210-11
GAGTACT464550.020.64269612-13
CAGAGTA666650.020.0769710-11
TGGTATC107650.019.5400872
AGTACTT486100.019.29696512-13
GTACTTT504750.018.66261714-15
Produced by FastQC (version 0.11.5)