FastQCFastQC Report
Thu 2 Jun 2016
DRR015338_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015338_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9744586
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253040.2596723965492223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207580.2130208507575386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115150.11816818077237967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157800.047.5473141
GTATCAA285050.037.8622131
ATCAACG333350.031.8600273
TCAACGC334750.031.753974
CAACGCA352850.030.1637885
TATCAAC359750.029.5725982
AACGCAG361600.029.5344056
GTACATG266800.027.2352521
ACGCAGA407300.025.907767
TACATGG268150.025.7766062
CGCAGAG410200.025.5689038
ACATGGG270050.024.4662933
GCAGAGT444550.023.048489
GTGGTAT81000.019.4358431
GAGTACT307600.019.39147612-13
AGAGTAC404550.019.35544410-11
CATGGGT82900.019.1565234
CATGGGG155250.019.0807364
CAGAGTA443700.019.05757710-11
GTACTTT326800.018.07111414-15
Produced by FastQC (version 0.11.5)