FastQCFastQC Report
Thu 2 Jun 2016
DRR015338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9744586
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258270.26503947935807637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183960.18878175019441565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142640.14637871737188218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145550.043.897441
GTATCAA279450.040.6883241
ATCAACG352550.032.030033
TCAACGC355650.031.635714
CAACGCA370900.030.2736365
TATCAAC376200.030.1813932
AACGCAG383850.029.34446
ACGCAGA444150.025.1453697
CGCAGAG445350.024.9245158
GTACATG244900.024.3028831
TACATGG246100.023.1238422
GCAGAGT476550.022.7145739
ACATGGG250950.021.7736913
AGAGTAC429150.020.9720710-11
AGTACTT340150.019.69096812-13
GAGTACT328900.018.61442212-13
CAGAGTA473200.017.60628710-11
TTTCGCG7300.017.4523439
CATGGGT80750.016.9594
CATGGGG140300.016.9273554
Produced by FastQC (version 0.11.5)