FastQCFastQC Report
Thu 2 Jun 2016
DRR015337_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015337_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12170096
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320500.26335042878872933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259180.21296463068163143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146930.12073035414018098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169000.052.903461
GTATCAA320200.041.336211
ATCAACG375150.034.690343
TCAACGC375850.034.601384
CAACGCA393750.033.0052765
TATCAAC404350.032.2753522
AACGCAG407850.031.8973126
ACGCAGA462750.027.8278967
CGCAGAG467950.027.3529078
GTACATG326350.027.1311021
TACATGG321900.025.9538022
ACATGGG327550.024.6169153
GCAGAGT510450.024.4491069
GTGGTAT74550.023.2533511
CAGAGTA501650.020.44748110-11
CATGGGG206300.020.0498144
GAGTACT369450.019.70393412-13
TGGTATC81100.019.4135322
AGAGTAC478150.019.41137910-11
GAGTACA242150.019.2784061
Produced by FastQC (version 0.11.5)