FastQCFastQC Report
Thu 2 Jun 2016
DRR015337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12170096
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328970.2703101109473582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246610.20263603508139952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172220.14151079827143517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163950.052.1842421
GTATCAA319300.042.8404121
ATCAACG391600.034.5503543
TCAACGC393400.034.3460164
CAACGCA407100.033.156655
TATCAAC419950.032.3191222
AACGCAG419650.032.3044136
ACGCAGA480450.027.8849547
CGCAGAG484400.027.5355938
GTACATG304800.027.1274431
TACATGG298950.026.2258632
ACATGGG306050.024.5270773
GCAGAGT536000.024.3384029
GTGGTAT73400.022.1696451
AGAGTAC501100.020.6488610-11
CAGAGTA524900.019.83396310-11
GAGTACT392700.019.59357512-13
TGGTATC79350.019.164382
CATGGGG190650.019.0662044
AGTACTT406150.018.9559212-13
Produced by FastQC (version 0.11.5)