FastQCFastQC Report
Thu 2 Jun 2016
DRR015336_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015336_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9394589
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284860.30321709656484175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242700.25834019987462997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141130.15022477300497128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139750.056.421861
GTATCAA264000.041.2936741
ATCAACG319600.033.5568543
TCAACGC320250.033.4745334
CAACGCA336150.031.782895
TATCAAC346900.031.0471942
AACGCAG347600.030.8406776
GTACATG322250.030.3702341
TACATGG313900.029.353652
ACATGGG321750.027.5909793
ACGCAGA395150.026.9452517
CGCAGAG401500.026.394298
CATGGGT109750.024.2123364
GCAGAGT435300.023.8519029
GTGGTAT53800.022.5802971
CATGGGG172550.021.7026754
GAGTACA231300.020.811851
CAGAGTA425800.020.60677310-11
AGAGTAC412700.019.80563510-11
GAGTACT308900.019.62661412-13
Produced by FastQC (version 0.11.5)