FastQCFastQC Report
Thu 2 Jun 2016
DRR015336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9394589
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283170.30141818870415726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228480.2432038272243735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162800.17329124243753505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131400.055.9242821
GTATCAA254050.041.7908361
ATCAACG326600.032.136543
TCAACGC326850.032.056284
CAACGCA338450.030.8769325
TATCAAC351650.030.1807482
GTACATG298900.029.8924081
AACGCAG350300.029.8697956
TACATGG291850.028.9077382
ACATGGG298700.026.7763463
ACGCAGA407100.025.4675877
CGCAGAG412100.025.0261178
CATGGGT106650.023.8888284
GCAGAGT450650.022.5162269
CATGGGG153000.020.8446354
GTGGTAT56400.020.2065281
AGAGTAC428350.020.0610710-11
CAGAGTA442150.019.2960110-11
TGGTATC57900.018.8725682
GAGTACA215750.018.868241
Produced by FastQC (version 0.11.5)