FastQCFastQC Report
Thu 2 Jun 2016
DRR015335_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015335_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9711814
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320750.3302678572715664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245840.2531349962015335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148470.15287566256932023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144300.057.5442431
GTATCAA288600.042.929581
ATCAACG345850.035.2215463
TCAACGC347450.035.03284
CAACGCA367000.033.1914065
TATCAAC368650.033.1419372
AACGCAG383800.031.8689446
CGCAGAG426700.028.2701558
ACGCAGA429500.028.2449197
GTACATG279550.026.3507331
GCAGAGT466550.025.2675659
TACATGG280800.024.777542
ACATGGG285250.023.5295833
GTGGTAT58400.023.2171421
GAGTACT321900.021.32142612-13
CAGAGTA453950.020.95742810-11
AGAGTAC427500.020.76941910-11
TGGTATC60300.020.4503062
CATGGGT97500.020.3014954
GTACTTT353400.019.17634814-15
Produced by FastQC (version 0.11.5)