FastQCFastQC Report
Thu 2 Jun 2016
DRR015335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9711814
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328400.33814486150579076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203540.20957979631817497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189350.19496872571900573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118650.053.80881
GTATCAA264900.047.359511
ATCAACG357150.034.75013
TCAACGC360400.034.398864
TATCAAC377350.032.9202462
CAACGCA377850.032.774125
AACGCAG392450.031.6110216
ACGCAGA455850.026.9750217
CGCAGAG454200.026.9428128
GTACATG239600.024.6491031
GCAGAGT493400.024.1799969
TACATGG241600.023.494892
AGAGTAC460550.022.29882410-11
ACATGGG249100.022.0990683
AGTACTT371750.020.69168512-13
CATGGGT85700.019.6949734
CAGAGTA485200.019.149519
GAGTACT356650.018.92678812-13
GTACTTT382200.017.46716914-15
TACTTTT456250.016.8913914-15
Produced by FastQC (version 0.11.5)