FastQCFastQC Report
Thu 2 Jun 2016
DRR015334_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015334_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8256320
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302370.3662285376535793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246790.2989104104492074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137010.16594560288360916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130100.055.221961
GTATCAA251400.042.2780081
ATCAACG300600.034.6947823
TCAACGC302600.034.5404474
TATCAAC313100.033.411792
CAACGCA317100.032.961025
AACGCAG324750.032.282656
ACGCAGA369150.028.2642257
CGCAGAG372050.027.9092148
GTACATG243950.027.2493631
TACATGG246250.026.0732142
GCAGAGT407750.024.9723789
ACATGGG245550.024.7203373
TATAACG5400.023.5943532
GTGGTAT56250.022.6486571
CAGAGTA392650.021.32671710-11
GAGTACT288000.020.85332312-13
AGAGTAC373900.020.33970610-11
TGGTATC60350.019.679182
CATGGGT65950.019.387754
Produced by FastQC (version 0.11.5)