FastQCFastQC Report
Thu 2 Jun 2016
DRR015334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8256320
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297960.36088717491570094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234140.28358881438703926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155000.18773497151273205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127050.055.6619721
GTATCAA247250.042.65411
ATCAACG312650.033.5698623
TCAACGC312650.033.5407564
CAACGCA325450.032.2495545
TATCAAC328650.032.0455782
AACGCAG334900.031.4329326
ACGCAGA388550.026.9639637
CGCAGAG393200.026.5988088
GTACATG235500.025.6341291
GTGGTAT52650.024.6812951
GCAGAGT426200.024.1067839
TACATGG238000.024.0291732
TATAACG6900.023.0964412
ACATGGG237250.022.895943
AGAGTAC396150.021.12296110-11
TGGTATC57650.020.6142392
CAGAGTA414250.020.32634410-11
GAGTACT305400.020.06943912-13
AGTACTT310850.019.82735612-13
Produced by FastQC (version 0.11.5)