FastQCFastQC Report
Thu 2 Jun 2016
DRR015333_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015333_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375677
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234340.31771998692459014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181870.24658075455310746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107390.14560019371781058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112500.053.8715131
GTATCAA215500.041.8469541
ATCAACG260300.034.140583
TCAACGC262350.034.0296754
CAACGCA274700.032.4666375
TATCAAC277900.032.109382
AACGCAG283550.031.5652126
ACGCAGA319200.027.8260087
GTACATG202350.027.4099351
CGCAGAG323000.027.3577698
TACATGG201650.025.9503022
ACATGGG204150.024.6295053
GCAGAGT353350.024.54229
TACCTGG59550.023.7641052
TATAACG8250.023.7168672
ATAACGC9000.023.2572383
TAACGCA8500.023.0191574
GTGGTAT52050.022.9027711
GTACCTG63850.022.731981
TCTGTCG16100.021.479388
Produced by FastQC (version 0.11.5)