FastQCFastQC Report
Thu 2 Jun 2016
DRR015333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375677
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234650.3181402873254889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174860.23707654226181543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120110.16284606823210943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107100.053.6765671
GTATCAA215100.042.854841
ATCAACG264250.034.4160423
TCAACGC265650.034.3202974
CAACGCA272750.033.393545
TATCAAC279450.032.8024562
AACGCAG283400.032.3455666
TATAACG6500.030.8252052
ACGCAGA329800.027.5879177
CGCAGAG334650.027.079348
TAACGCA9050.026.1407414
ATAACGC8800.025.8493983
GCAGAGT361800.024.5618789
TACCTGG60650.024.5518152
GTACATG184900.024.3601041
GTACCTG65750.022.8117831
TACATGG186050.022.7869742
ACATGGG186200.021.5502363
AGAGTAC338050.020.93053210-11
GTGGTAT49600.020.1289711
Produced by FastQC (version 0.11.5)