FastQCFastQC Report
Thu 2 Jun 2016
DRR015332_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015332_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9683027
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326540.3372292569255461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261750.2703183622228875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148150.15299967665070024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169000.051.718231
GTATCAA314700.040.90151
ATCAACG369600.034.1526683
TCAACGC371150.034.0589034
CAACGCA385600.032.664575
TATCAAC396800.032.0065082
AACGCAG398650.031.7664976
ACGCAGA450800.027.9200487
CGCAGAG455600.027.4954858
GTACATG291800.025.6497781
GCAGAGT492700.024.9884479
TACATGG288500.024.5422212
ACATGGG297900.022.866593
GTGGTAT78650.021.9830471
CAGAGTA478450.021.14911810-11
GAGTACT341950.020.94930512-13
AGAGTAC456450.020.03036510-11
TGGTATC83500.019.83652
GTACTTT363600.019.43287814-15
AGTACTT348150.018.70091212-13
Produced by FastQC (version 0.11.5)