FastQCFastQC Report
Thu 2 Jun 2016
DRR015332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9683027
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336150.34715383939340455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247270.2553643607520665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180610.1865222517710629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153400.050.6072461
GTATCAA299900.043.3659361
ATCAACG382350.033.7687453
TCAACGC383350.033.6450584
CAACGCA395100.032.632965
TATCAAC404900.032.0798032
AACGCAG408200.031.740266
ACGCAGA474450.027.1356037
CGCAGAG477750.026.7862878
GCAGAGT510350.024.6600999
GTACATG267000.024.397451
TACATGG267200.023.0876962
ACATGGG273750.021.3722633
AGAGTAC477350.021.34663410-11
AGTACTT374150.020.15707412-13
GAGTACT366450.019.6245412-13
CAGAGTA503800.019.52597610-11
GTACTTT390700.018.25687614-15
GTGGTAT72800.017.9710711
TATACTG48300.017.5198145
Produced by FastQC (version 0.11.5)