FastQCFastQC Report
Thu 2 Jun 2016
DRR015331_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015331_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12921493
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203940.15783005880202852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177970.13773176211139068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191450.051.286891
GTATCAA352350.039.5199851
ATCAACG427500.031.9982813
TCAACGC430500.031.7547664
TATCAAC447200.030.7462732
CAACGCA457600.029.874885
AACGCAG487850.028.125086
CGCAGAG521700.025.6305628
ACGCAGA529900.025.447
GTGGTAT87950.024.3261621
GTACATG363200.024.0764871
TACATGG364350.022.7925742
GCAGAGT573500.022.6265859
ACATGGG369350.021.7039893
TGGTATC95850.021.470082
GAGTACT353700.019.5849212-13
AGAGTAC521800.019.49712810-11
CAGAGTA555950.019.0278710-11
AGTACTT375100.017.78830312-13
CATGGGG224800.017.617724
Produced by FastQC (version 0.11.5)