FastQCFastQC Report
Thu 2 Jun 2016
DRR015331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12921493
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247110.19123951079027787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217030.1679604671070131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211250.052.607761
GTATCAA388300.039.775631
ATCAACG470600.032.354713
TCAACGC474500.032.1175464
TATCAAC497950.030.7823052
CAACGCA495500.030.7563575
AACGCAG523400.029.1255726
CGCAGAG577600.025.7707218
ACGCAGA583250.025.7550747
GTGGTAT92800.025.3746031
GTACATG375750.024.5703581
TACATGG378700.023.2112052
GCAGAGT629050.023.0411649
TGGTATC97700.022.4925352
ACATGGG383750.021.9546413
GAGTACT400850.020.48252712-13
AGAGTAC572800.019.8842610-11
CAGAGTA609550.019.75647410-11
AGTACTT422700.018.62721412-13
GTACTTT439550.018.37559714-15
Produced by FastQC (version 0.11.5)