FastQCFastQC Report
Thu 2 Jun 2016
DRR015330_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015330_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490966
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126740.1014653310240377No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198300.039.489281
GTATCAA329850.035.11351
ATCAACG381900.029.5874963
TCAACGC385100.029.3774574
TATCAAC410450.027.7788162
CAACGCA409850.027.6042985
AACGCAG428000.026.5613426
GTACATG372200.024.0847851
ACGCAGA469000.023.7858267
CGCAGAG472850.023.2749258
TACATGG368600.023.0142672
ACATGGG375200.021.6707863
GCAGAGT516200.020.7400269
AGAGTAC434850.018.57747310-11
GAGTACT269700.018.23649812-13
CAGAGTA504550.017.30067410-11
CATGGGG223750.017.0303554
GTACTTT291650.016.55195214-15
GAGTACA293600.016.530071
AGTACTT284850.016.28424812-13
Produced by FastQC (version 0.11.5)