FastQCFastQC Report
Thu 2 Jun 2016
DRR015330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490966
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155320.12434586724517542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135960.1088466656622074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232500.038.923431
GTATCAA378400.033.195511
ATCAACG440700.028.0461433
TCAACGC443050.027.8460454
CAACGCA459850.026.8188345
TATCAAC465850.026.6220262
AACGCAG480450.025.7920286
GTACATG382650.023.066851
ACGCAGA537600.022.5513847
CGCAGAG538800.022.239428
TACATGG380850.022.1590582
ACATGGG389000.020.7341253
GCAGAGT582700.020.0410889
GAGTACT310650.018.83526812-13
AGAGTAC486150.018.65113610-11
CATGGGG230800.017.5026474
GTACTTT331650.017.36301814-15
CAGAGTA569800.017.07877210-11
AGTACTT328400.016.95822712-13
GAGTACA305950.016.2111431
Produced by FastQC (version 0.11.5)