FastQCFastQC Report
Thu 2 Jun 2016
DRR015329_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015329_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10070576
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212870.2113781773753557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188240.18692078784768618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105670.10492944991428493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173800.054.931091
GTATCAA328200.040.200311
ATCAACG400900.032.4633753
TCAACGC407400.031.9353684
TATCAAC417250.031.3613362
CAACGCA428200.030.4061095
AACGCAG447100.029.263326
GTGGTAT78450.028.146111
TGGTATC78200.026.8409772
ACGCAGA493150.026.1541947
CGCAGAG488750.026.1109128
GTACATG345750.024.76831
TACATGG346700.023.8093812
GCAGAGT525850.023.6129369
ACATGGG349250.022.5358623
GAGTACT324150.020.47270612-13
CAGAGTA518650.019.94476510-11
AGAGTAC490400.019.86899810-11
GTACTTT350000.018.75227414-15
AGTACTT339950.018.52404612-13
Produced by FastQC (version 0.11.5)