FastQCFastQC Report
Thu 2 Jun 2016
DRR015329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10070576
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248810.24706630484691242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225430.2238501551450483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129170.12826475863942638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194100.055.0060161
GTATCAA354500.039.6513331
ATCAACG437400.031.7318673
GTGGTAT77200.031.4476341
TCAACGC443650.031.3258574
TATCAAC455000.030.6289792
CAACGCA459550.030.2816095
AACGCAG483050.028.8272656
TGGTATC82150.028.3569362
ACGCAGA537100.025.5964747
CGCAGAG534400.025.4959538
GTACATG359600.024.357781
TACATGG356350.023.44192
GCAGAGT574400.023.2932479
ACATGGG361800.022.129743
GAGTACT371700.020.35037812-13
CAGAGTA567200.020.18087810-11
AGAGTAC539600.019.76714310-11
GTACTTT393350.018.91360514-15
AGTACTT384450.018.47423412-13
Produced by FastQC (version 0.11.5)