FastQCFastQC Report
Thu 2 Jun 2016
DRR015328_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015328_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7277029
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190000.26109556523685695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172660.2372671594410301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97710.13427182989101735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205200.034.788011
GTATCAA318700.030.9840621
ATCAACG382100.025.5302583
TCAACGC385200.025.3486044
TATCAAC394500.024.8377862
CAACGCA398100.024.5509265
GTACATG220300.024.178661
AACGCAG413600.023.7849486
TACATGG220250.023.3397242
ACGCAGA449350.021.7078637
CGCAGAG449600.021.5945998
ACATGGG227100.021.4074123
TATAACG8600.021.177722
AGAGTAC378700.020.51531810-11
GCAGAGT471250.020.198699
TACCTGG51100.019.9592742
GAGTACT287100.019.56445712-13
AGTACTT294450.018.80567412-13
TAACGCA10800.018.5456474
GTACCTG59550.018.1951681
Produced by FastQC (version 0.11.5)