FastQCFastQC Report
Thu 2 Jun 2016
DRR015328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7277029
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217520.2989131965806375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203950.2802654764739841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117720.16176931547201473No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76550.10519402904674421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232700.035.499911
GTATCAA353350.030.2091771
ATCAACG425150.024.6845933
TCAACGC426600.024.6326834
TATCAAC434950.024.371482
TACCTGG54250.024.3200022
CAACGCA437100.024.072185
AACGCAG452100.023.364066
GTACATG216350.023.1958891
GTACCTG61350.022.639751
TACATGG215100.021.9755862
TATAACG5950.021.4094942
ACGCAGA492750.021.1326857
CGCAGAG494350.020.9722638
ACATGGG224850.020.2921753
AGAGTAC412250.020.12444510-11
GAGTACT322200.020.1170112-13
GCAGAGT517850.019.6696919
AGTACTT325900.018.78563712-13
TCTGTCG18650.018.5383158
Produced by FastQC (version 0.11.5)