FastQCFastQC Report
Thu 2 Jun 2016
DRR015327_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015327_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590034
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103060.2245299272292972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98630.21487858259873457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.1121560319596761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114800.046.48261
GTATCAA184150.033.823541
ATCAACG223800.027.5654373
TCAACGC224650.027.3601074
TATCAAC230200.026.8447742
CAACGCA234250.026.297725
AACGCAG247400.024.918336
GTGGTAT55750.023.1941341
CGCAGAG259650.023.1130898
ACGCAGA269150.022.6689387
TGGTATC58050.022.0439452
GCAGAGT270800.021.4902949
GTACATG150900.020.7890511
GAGTACT168050.020.21123112-13
TACATGG148150.020.103022
GCGGTAT3650.019.9586771
CAGAGTA267050.019.5685610-11
GTACTTT175350.018.87681414-15
AGAGTAC242900.018.84477410-11
ACATGGG154600.018.229173
Produced by FastQC (version 0.11.5)