FastQCFastQC Report
Thu 2 Jun 2016
DRR015327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590034
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120980.26357103237143775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120150.26176276689889444No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT105200.2291922020621198No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT67510.14707952054385653TruSeq Adapter, Index 1 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63080.13742817591329387No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG61440.1338552176301962TruSeq Adapter, Index 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123300.047.321971
GTATCAA206000.035.4661371
ATCAACG252850.028.6236763
TCAACGC256100.028.2781934
TATCAAC258400.028.0845032
CAACGCA264950.027.2821245
AACGCAG278350.026.1158226
CGCAGAG295650.023.7732018
ACGCAGA307050.023.3794887
GTGGTAT59700.022.431061
GCAGAGT311800.022.0022479
TGGTATC61750.020.92572
GAGTACT196400.020.61937712-13
GTACATG158500.020.5472431
TACATGG155750.020.1274972
CAGAGTA305200.019.72963510-11
AGAGTAC277100.019.34963410-11
GTACTTT207150.019.22111114-15
ACATGGG160550.018.785443
AGTACTT200100.018.65771712-13
Produced by FastQC (version 0.11.5)