FastQCFastQC Report
Thu 2 Jun 2016
DRR015326_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015326_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11770691
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204320.173583691900501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180920.15370380549451176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA319650.030.8046631
GTATCAA481000.029.4624391
ATCAACG558700.025.0382373
TCAACGC560650.024.9595894
TATCAAC582000.024.1104472
CAACGCA581350.024.0715755
AACGCAG602050.023.3422346
GTACATG372700.023.2808131
TACATGG370650.022.4424462
ACGCAGA653850.021.2778637
CGCAGAG654050.021.1326878
ACATGGG383700.020.7855823
GAGTACT354650.019.70575712-13
AGAGTAC527750.019.53590610-11
GCAGAGT695200.019.3527839
GTACTTT375650.018.33756414-15
AGTACTT369600.017.89307412-13
CATGGGG228950.017.3778554
GAGTACA293650.016.5877861
CAGAGTA683600.016.13364410-11
Produced by FastQC (version 0.11.5)