FastQCFastQC Report
Thu 2 Jun 2016
DRR015326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11770691
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248250.21090520514046285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222190.1887654683994338No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT124910.1061195132894067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118580.10074174914624807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA358550.032.064041
GTATCAA527650.028.4697271
ATCAACG612650.024.0734483
TCAACGC615850.023.9705224
GTACATG382400.023.4057881
CAACGCA631900.023.368885
TATCAAC635900.023.3458822
AACGCAG653850.022.6401336
TACATGG381000.022.41412
ACATGGG391700.020.9169273
ACGCAGA717950.020.3781687
CGCAGAG719600.020.167078
GAGTACT391400.019.96536812-13
AGAGTAC565050.019.23527510-11
GCAGAGT760100.018.7337329
GTACTTT416250.018.44657914-15
AGTACTT403900.018.11396812-13
CATGGGG228350.017.6908914
GAGTACA299150.017.2207571
CAGAGTA746350.016.33658810-11
Produced by FastQC (version 0.11.5)