FastQCFastQC Report
Thu 2 Jun 2016
DRR015325_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015325_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10949965
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229470.20956231366949574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211470.1931239049622533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA315700.033.6998251
GTATCAA472300.030.3720381
ATCAACG547050.025.9045313
TCAACGC552300.025.699974
TATCAAC568650.025.0294322
CAACGCA571850.024.8940325
AACGCAG593400.024.0360056
GTACATG341600.023.1353111
TACATGG336650.022.3157962
CGCAGAG642300.021.6831678
ACGCAGA648900.021.6159927
ACATGGG344000.021.1334383
GAGTACT358350.021.07060612-13
GCAGAGT676850.020.0861829
AGAGTAC521300.019.53793110-11
GTACTTT387600.019.14557814-15
AGTACTT376550.018.14907512-13
CATGGGG186000.017.230244
CAGAGTA669750.017.18087610-11
GAGTACA273950.015.9198041
Produced by FastQC (version 0.11.5)