FastQCFastQC Report
Thu 2 Jun 2016
DRR015325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10949965
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271170.24764462717460742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251980.23011945700283062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131830.12039307888198729No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117180.1070140406841483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA335450.033.726211
GTATCAA512150.030.440621
ATCAACG600650.025.5307253
TCAACGC606600.025.2877984
CAACGCA621100.024.7120885
TATCAAC623650.024.6799582
AACGCAG648450.023.7469626
GTACATG343550.023.0013481
TACATGG338450.022.3418872
ACGCAGA715100.021.2288847
CGCAGAG711250.021.17758
ACATGGG341350.021.0163693
GAGTACT416950.020.46643812-13
GCAGAGT745550.019.7764349
AGAGTAC572000.019.76504710-11
GTACTTT443050.018.90244714-15
AGTACTT433000.018.23668512-13
CATGGGG192400.017.472914
CAGAGTA738200.016.79730610-11
TATAACG9550.016.1979082
Produced by FastQC (version 0.11.5)