FastQCFastQC Report
Thu 2 Jun 2016
DRR015324_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015324_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11354634
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247780.21821927505545313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220470.19416742098424308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124540.10968209102997067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200350.054.285621
GTATCAA363350.039.4678461
ATCAACG444450.031.6382753
TCAACGC448650.031.3222184
TATCAAC471500.030.0718482
CAACGCA470400.029.913075
GTGGTAT86500.029.2628571
AACGCAG488600.028.919956
TGGTATC86750.027.3458482
ACGCAGA542250.025.706837
CGCAGAG545500.025.3961078
GTACATG346200.023.354681
GCAGAGT581900.023.0973229
TACATGG346950.022.2315462
ACATGGG352000.020.8468363
CAGAGTA576600.020.11777110-11
GAGTACT393350.019.8028612-13
AGAGTAC550350.019.58509310-11
GTACTTT417750.018.28651214-15
AGTACTT405800.017.91150912-13
Produced by FastQC (version 0.11.5)