FastQCFastQC Report
Thu 2 Jun 2016
DRR015324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11354634
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294660.2595063830326896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270360.2381054290257176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151550.13346973579245267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212700.055.241951
GTATCAA395300.041.0262641
ATCAACG493600.032.5341843
TCAACGC501050.032.077684
TATCAAC512250.031.476372
CAACGCA517250.031.0554315
AACGCAG535750.030.0764626
GTGGTAT84300.029.9834921
TGGTATC84650.028.5928552
ACGCAGA600950.026.5035277
CGCAGAG604050.026.14918
GCAGAGT646550.023.9448249
GTACATG352850.022.3937151
TACATGG356050.021.4031012
ACATGGG357550.020.5610353
GAGTACT451700.020.46326812-13
CAGAGTA639150.020.31493810-11
AGAGTAC610950.019.97180710-11
GTACTTT471600.019.31678614-15
AGTACTT460650.018.81127412-13
Produced by FastQC (version 0.11.5)