FastQCFastQC Report
Thu 2 Jun 2016
DRR015323_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015323_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10683907
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254680.23837721537635997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229080.21441594353077015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128090.11989059807428126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244150.045.1066861
GTATCAA409450.035.5359271
ATCAACG493950.029.1320293
TCAACGC499300.028.8292644
TATCAAC511600.028.1075342
CAACGCA520550.027.6352885
AACGCAG543200.026.608686
ACGCAGA593900.023.9395287
CGCAGAG590900.023.8996038
GTACATG352700.023.1568131
TACATGG350800.022.1831742
GCAGAGT631450.021.817969
ACATGGG358100.020.911773
GAGTACT387550.020.26330412-13
AGAGTAC548750.019.7291510-11
GTGGTAT129450.019.2022611
CAGAGTA618950.018.97639710-11
GTACTTT415350.018.63306414-15
TGGTATC127950.018.4700572
AGTACTT399000.018.37045312-13
Produced by FastQC (version 0.11.5)