FastQCFastQC Report
Thu 2 Jun 2016
DRR015323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10683907
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300330.28110503021039024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274930.2573309558010941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155370.1454243283847379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263900.043.715521
GTATCAA443300.036.04141
ATCAACG547850.028.8707563
TCAACGC551400.028.668384
TATCAAC562250.028.207982
CAACGCA567800.027.840345
AACGCAG593300.026.7204366
ACGCAGA657450.023.8995087
CGCAGAG653200.023.8878638
GTACATG357850.023.4310931
TACATGG354200.022.3755472
GCAGAGT694800.021.9472269
ACATGGG361050.021.0786953
AGAGTAC601350.020.23738110-11
GAGTACT440300.020.20252612-13
AGTACTT453050.018.9359912-13
GTGGTAT142650.018.9012171
GTACTTT466500.018.85965214-15
CAGAGTA685100.018.71638110-11
TCGCGCG1500.001032842218.1968159
Produced by FastQC (version 0.11.5)