FastQCFastQC Report
Thu 2 Jun 2016
DRR015322_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015322_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12106047
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196260.16211732863749825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164680.13603119168461844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202900.049.3114971
GTATCAA368500.039.158341
ATCAACG445950.031.84813
TCAACGC450150.031.5824644
TATCAAC466650.030.6838022
CAACGCA477800.029.7837585
AACGCAG510300.027.9493356
CGCAGAG539150.025.5610398
ACGCAGA546500.025.4165447
GTACATG357950.023.7169591
GCAGAGT583200.022.8981789
TACATGG359900.022.5679862
GTGGTAT100950.022.4105551
ACATGGG367100.021.1052383
TGGTATC100450.021.0757392
AGAGTAC528550.019.3560710-11
CAGAGTA564450.019.32606910-11
GAGTACT354800.019.19102312-13
GTACTTT364700.018.12718414-15
TCTGTCG26700.017.8923288
Produced by FastQC (version 0.11.5)