FastQCFastQC Report
Thu 2 Jun 2016
DRR015322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12106047
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225090.18593187355046614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194810.16091958010736288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209300.051.5707631
GTATCAA384900.038.9052621
ATCAACG465050.031.9481413
TCAACGC470350.031.5881444
TATCAAC486400.030.727322
CAACGCA488400.030.3741575
AACGCAG520250.028.5321226
CGCAGAG567950.025.415258
ACGCAGA577100.025.2803087
GTGGTAT98800.023.7396741
TGGTATC98750.022.9443172
GCAGAGT614850.022.8108989
GTACATG369800.022.4021761
TACATGG364250.021.7336442
ACATGGG370550.020.4775663
GAGTACT383400.019.57484412-13
CAGAGTA597550.019.3878610-11
AGAGTAC559100.019.26464310-11
GTACTTT391050.018.7109114-15
AGTACTT388100.018.32371312-13
Produced by FastQC (version 0.11.5)