FastQCFastQC Report
Thu 2 Jun 2016
DRR015321_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015321_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11388444
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259250.22764303885587883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227380.19965853105130077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126660.11121800309155491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210400.055.5703661
GTATCAA397300.039.7653581
ATCAACG493350.031.6978153
TCAACGC498800.031.360744
TATCAAC515100.030.55172
CAACGCA523650.029.8818555
AACGCAG548800.028.6700736
GTGGTAT91600.028.4311581
TGGTATC87700.027.8822562
CGCAGAG595700.025.9300068
ACGCAGA601850.025.9213877
GTACATG406250.024.4543081
TACATGG401350.023.9053152
GCAGAGT641950.023.67369
ACATGGG413300.022.4710043
CAGAGTA635650.020.26473210-11
GAGTACT421450.020.06448212-13
AGAGTAC607800.019.92063710-11
CATGGGT101100.019.2731294
GTACTTT440850.018.94880314-15
Produced by FastQC (version 0.11.5)