FastQCFastQC Report
Thu 2 Jun 2016
DRR015321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11388444
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308460.27085350729212876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275460.24187676560555593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156420.1373497555943551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227600.056.133181
GTATCAA430950.040.246591
ATCAACG534350.032.1647383
TCAACGC538100.031.9236764
TATCAAC555750.031.0845852
CAACGCA557550.030.8181865
TGGTATC89750.030.1624412
GTGGTAT95100.029.646211
AACGCAG584500.029.451716
CGCAGAG644900.026.2217258
ACGCAGA651200.026.1357127
GTACATG425650.024.0013481
GCAGAGT698550.023.817089
TACATGG419700.023.252642
ACATGGG427550.022.036223
GAGTACT463350.020.42017612-13
CAGAGTA694900.020.32579410-11
AGAGTAC664450.019.82277510-11
GTACTTT477200.019.5578314-15
AGTACTT469000.018.9954512-13
Produced by FastQC (version 0.11.5)