FastQCFastQC Report
Thu 2 Jun 2016
DRR015320_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015320_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682958
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288230.21064889624012587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260640.1904851275579447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146860.10733059328253437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232700.053.504541
GTATCAA421000.039.9325371
ATCAACG520900.031.9949053
TCAACGC528150.031.5472074
TATCAAC544600.030.697012
CAACGCA552750.030.1681275
AACGCAG581450.028.7729746
GTGGTAT103300.027.6310211
ACGCAGA647650.025.4007237
CGCAGAG653750.025.019048
TGGTATC114400.023.360552
GTACATG461700.023.3579581
GCAGAGT701900.022.7008329
TACATGG467700.021.950792
ACATGGG471700.020.7824483
CAGAGTA673550.020.04242110-11
GAGTACT431050.019.83331312-13
AGAGTAC648650.019.60180910-11
GTACTTT450400.018.68828614-15
AGTACTT443400.017.90075712-13
Produced by FastQC (version 0.11.5)