FastQCFastQC Report
Thu 2 Jun 2016
DRR015320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682958
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337710.24681066769334528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310730.22709270904726886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175760.1284517572881536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256750.054.786661
GTATCAA461450.039.851261
ATCAACG572200.031.6992473
TCAACGC579000.031.295534
TATCAAC594000.030.7302672
CAACGCA596200.030.4155675
GTGGTAT102400.029.4486921
AACGCAG630300.028.8350096
ACGCAGA708350.025.4137487
TGGTATC114850.025.0763632
CGCAGAG715850.024.9568338
GCAGAGT758200.023.0050759
GTACATG459450.022.664491
TACATGG463200.021.560452
CAGAGTA734850.020.38736310-11
GAGTACT493400.020.28323712-13
ACATGGG468100.020.1956353
AGAGTAC707350.019.6974410-11
GTACTTT510250.019.23181214-15
AGTACTT505200.018.26489312-13
Produced by FastQC (version 0.11.5)