FastQCFastQC Report
Thu 2 Jun 2016
DRR015319_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015319_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11573771
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356000.3075920544825019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321350.277653670527955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186760.16136486543582035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239550.060.1006621
GTATCAA460950.040.9533161
GTGGTAT91550.034.1674041
TGGTATC89050.033.9522362
ATCAACG575400.032.3922923
TCAACGC583500.031.9816344
TATCAAC593500.031.5629672
CAACGCA609850.030.6596325
AACGCAG636550.029.5666736
ACGCAGA704300.026.4072387
CGCAGAG700400.026.3929148
GTACATG461850.025.1491371
GCAGAGT743100.024.4970389
TACATGG460100.024.3157232
ACATGGG479400.022.3683973
CAGAGTA738200.021.26063710-11
GAGTACT499400.020.8812512-13
GTACTTT509750.020.10021214-15
AGAGTAC715600.019.9769910-11
CATGGGG227900.019.4723284
Produced by FastQC (version 0.11.5)