FastQCFastQC Report
Thu 2 Jun 2016
DRR015319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11573771
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402570.3478295881264628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372230.32161514168545413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211390.18264574268835973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251800.061.0731
GTATCAA484900.040.6572151
TGGTATC86750.038.4952552
GTGGTAT90400.038.3458861
ATCAACG613900.031.7532963
TCAACGC625000.031.167524
TATCAAC633300.030.9490172
CAACGCA642850.030.3091685
AACGCAG670200.029.1944716
ACGCAGA748300.025.8313167
CGCAGAG748050.025.6575078
GTACATG464200.024.6303831
TACATGG457150.023.9650522
GCAGAGT789400.023.8301689
ACATGGG478350.022.1587733
CAGAGTA783200.020.78714810-11
GAGTACT546100.020.3963612-13
AGAGTAC753800.020.17654210-11
GTACTTT549700.020.00356314-15
AGTACTT543700.019.3566312-13
Produced by FastQC (version 0.11.5)