FastQCFastQC Report
Thu 2 Jun 2016
DRR015318_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015318_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14211385
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304860.21451814865335084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272110.1914732448667037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA391250.035.5631
GTATCAA601700.031.485971
ATCAACG711000.026.2213523
TCAACGC714350.026.1304284
TATCAAC736900.025.464032
CAACGCA739250.025.3060515
AACGCAG766600.024.4388316
GTACATG434350.022.1282861
ACGCAGA839700.021.9373877
CGCAGAG841350.021.6137148
TACATGG426300.021.2447932
GCAGAGT878000.020.1008349
GAGTACT488700.019.67619312-13
ACATGGG439800.019.5601083
AGAGTAC697800.019.20185310-11
GTACTTT493800.018.97544114-15
AGTACTT486850.018.2557412-13
CAGAGTA863950.017.28114910-11
TCTGTCG31700.016.0742518
CATGGGG227800.016.0046654
Produced by FastQC (version 0.11.5)