FastQCFastQC Report
Thu 2 Jun 2016
DRR015318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14211385
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346230.24362861184888032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316750.2228846801349763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163840.11528784843982483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA429450.035.398341
GTATCAA658250.031.2330021
ATCAACG781850.026.0211123
TCAACGC787750.025.8724274
TATCAAC808350.025.2495232
CAACGCA809700.025.1710535
AACGCAG838600.024.3524286
GTACATG440100.022.4723111
ACGCAGA924050.021.7903147
TACATGG432500.021.6602572
CGCAGAG924950.021.5674538
GCAGAGT961350.020.136149
ACATGGG443050.019.9408663
GAGTACT560300.019.80617512-13
AGAGTAC756700.019.45351410-11
GTACTTT571700.019.04242314-15
AGTACTT563650.018.25561712-13
CAGAGTA944200.017.26245710-11
CATGGGG239650.016.0218474
GAGTACA366450.015.9869461
Produced by FastQC (version 0.11.5)